1. The amino acid sequence for the protein lysozyme is given below. Estimate the isoelectric point for lysozyme protein. The pK, values are provided in Table 3.1. KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNT DGSTDYGILQINSRWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKK IVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL Here's the sequence in this form: LYS VAL PHE GLY ARG CYS GLU LEU ALA ALA ALA MET LYS ARG HIS GLY
1. The amino acid sequence for the protein lysozyme is given below. Estimate the isoelectric point for lysozyme protein. The pK, values are provided in Table 3.1. KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNT DGSTDYGILQINSRWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKK IVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL Here's the sequence in this form: LYS VAL PHE GLY ARG CYS GLU LEU ALA ALA ALA MET LYS ARG HIS GLY
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:1.
The amino acid sequence for the protein lysozyme is given below. Estimate the isoelectric
point for lysozyme protein. The pK, values are provided in Table 3.1.
KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNT
DGSTDYGILQINSRWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKK
IVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL
Here's the sequence in this form:
LYS VAL PHE GLY ARG CYS GLU LEU ALA ALA ALA MET LYS ARG
HIS GLY
Table 3.1 Typical pk, values of ionizable groups in proteins
Group
Acid
Typical pK,
Base
Terminal a-carboxyl
3.1
group
Aspartic acid
Glutamic acid
4.1
N.
Histidine
6.0
-N
+ H
Terminal a-amino group
8.0
Cysteine
8.3
Тутosine
10.9
+ H
Lysine
10.8
H
H.
+ N-H
Arginine
12.5
N-H
N-H
Note: Values of pk, depend on temperature, ionic strength, and the microenvironment of the ionizable group.
in
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