0 10 20 30 40 50 60 70 temperature / °C a. What is the optimal temperature for this enzyme? b. What will happen to the enzyme's structure in temperatures well above and well below the optimal temperature. How will this affect the enzyme's function? rate of reaction
Q: An experiment on enzyme-catalyzed reaction was conducted in the laboratory by a student. Results…
A: One of the important models in enzyme kinetics to calculate the rate of enzymatic reaction is the…
Q: What is activation energy?
A: Enzymes are protein molecules which acts as a catalyst and speed up the reaction when they act on…
Q: 10. Analyze the figure on #10 of your hard copy of this test. Which will correctly explain how the…
A: The substrate is a substance where an enzyme functions in biochemistry. Chemical processes…
Q: An enzyme catalyzes the conversion of mannose to glucose. The KM of the enzyme is 0.135 μM and the…
A: Enzymes are biocatalyst that accelerates the rate of a chemical reaction. They themselves are not…
Q: p. The forward reaction would be written as: Please select the correct letters from graph (a-e) to…
A: Enzymes are biological catalyst. It catalysed all biological reactions that maintains homeostasis.…
Q: HN Histidine NH₂ OH HN + H |+ H-NH H
A: Enzymes are highly specialized proteins that have extraordinary catalytic power, greater than that…
Q: In First order, the rate of reaction would be . ., if the concertation of an enzyme is increased by…
A: The reaction may be classified according to the order of reaction, which is the number of reacting…
Q: Curve A (red), is enzyme catalyzed reaction treated with a compound. Curve B (blue), is the same…
A: Inhibitors are compound which inhibits the activity of activity of enzyme reversibly. This can be…
Q: What is the expected and logical result that you anticipate after you boil the extracted enzyme?…
A: There are several factors at which enzyme functioning is controlled. These factors include…
Q: Which result occurs when an enzyme-catalyzed reaction is heated to a few degrees above its maximum…
A: Enzymes are biocatalyst. Enzyme speed up the reaction.
Q: C3. Which of the following is NOT true about enzymes A. they accelerate the reverse rate of a…
A: Enzymes are biological catalysts that alter the rate of a reaction by lowering the activation…
Q: An enzyme catalyzes the reaction A ⇌ B. The enzyme is present at a concentration of 2 nM, and the…
A: Michaelis-Menten equation is used to calculate the rate at which a product is formed during a…
Q: Explain why enzyme activity varies with temperature, as shown here.
A: Enzyme activity is a measure of number of moles of product formed per unit time.
Q: 10 20 30 40 50 60 70 80 Temperature (°C)
A: Enzyme activity is influenced by many factors such as temperature, pH, concentration of the…
Q: 3. 4. 2 3 4. 5 6 7 8 pH of reaction According to Figure 1.7 which enzyme is most likely found in a…
A: The enzymes are biomolecules that catalyze biochemical reactions. These enzymes are very sensitive…
Q: Which factor leading to increased reaction rates for enzyme catalyzed reactions involves removing…
A: Short, strong or low barrier hydrogen bonds are found when the pK values of the atoms sharing the…
Q: You have two different enzymes, each with 2 substrates, S1 and S2, and 2 products, P1 and P2. A.…
A: There are three major reaction mechanisms seen with enzymes that catalyze a reaction involving 2…
Q: A series of enzymes catalyze the reaction A→B C→D. D binds to the enzyme that converts A to B at a…
A: Enzymes are biocatalyst, they increase the speed of a reaction by lowering its activation energy.…
Q: What would happen if you increased the concentration of the enzyme twofold? a) Vmax would also…
A: Enzymes are biocatalyst that increases the speed of reaction by lowering the activation…
Q: 42. A student conducts an experiment to test the efficiency of a certain enzyme. Which of the…
A: Enzymes are the proteins with catalytic powers.
Q: The enzyme can affect the following factors in a reaction: O a. The forward and reverse rates of the…
A: Enzymes are the biomolecules that can act as catalyst in the biochemical reactions and can increase…
Q: 5. Mary's husband died from a car accident while on a business trip. Because of this, she took to…
A: The intake of alcohol affects our bodies severely, it interfaces with many metabolic pathways…
Q: Which of the following statements about enzymatic catalysis is not true? O a. Enzymes are able to…
A: Enzymes are defined as complex compounds containing nitrogen. Catalysis is defined as a phenomenon…
Q: Lactose--->galactose+glucose represents an enzymatic reaction. What statement about this reaction is…
A: Lactase, also known as lactase-phlorizin hydrolase, is an enzyme found in animals' small intestine…
Q: 5. Examine the graphic below and answer the questions about the enzyme we reviewed in lecture. 1.0…
A: Enzymes are the biocatalysts that catalyze biochemical reactions by converting substrate bound to…
Q: You boiled the purified amylase from barely seeds extract and you mixed the boiled enzyme with…
A: The main function of Amylases is to hydrolyze the glycosidic bonds in starch molecules, converting…
Q: 8. Optimum (BEST) temperature for the action of this enzyme (graph below) is approximately 1. 50 1.…
A: Enzymes are very specific for their substrates and reaction conditions. These function at a specific…
Q: As you change the pH from a low pH to a high pH what happens to the rate of an enzymatic reaction,…
A: Enzyme are proteinaceous substances that increased reaction process . It contain active site to…
Q: VII. Graph the details on the unknown enzyme then answer the questions below: Enzyme A Enzyme B pH…
A: Enzymes exist in all biological systems and are important in regulating many biochemical reactions.…
Q: Heating an enzyme makes it no longer function correctly because the heat: O A) removes phosphate…
A: Enzymes are highly specialized proteins that have extraordinary catalytic power, greater than that…
Q: ion of estion udy As you increase the amount of substrate in a reaction (while keeping the enzyme…
A: Enzymes are the biocatalyst that are responsible for reducing the activation energy. The product…
Q: FollaVi m o ba A 20 40 Temperature (°C)
A: Enzymes are the biocatalyst that increases the rate of biochemical reaction by lowering the…
Q: Choose the wrong statement: Select one: O a. Competitive inhibitors competing with the substrate O…
A: Enzymes are the biological catalysts, that increase the rate of a chemical reaction. The enzymes may…
Q: Curve A (red), is enzyme catalyzed reaction treated with a compound. Curve B (blue), is the same…
A: The given graph represents the Lineweaver-Burk plot. Lineweaver-Burk plot is the plot between 1/V…
Q: When you heat a solution past the optimum for an enzyme? A. the active site permanently binds…
A: * Enzyme acts as catalyst by decreasing activation energy. * The enzymes will convert the substrate…
Q: Which of the following factors can denature an enzyme? 1. Competetive inhibitors 2.Extreme pH…
A: Enzymes are biochemical catalysts that function to increase the speed of biological reactions. They…
Q: Most enzymes are very sensitive to even small changes in pH. Explain why a slight shift to more…
A: Enzymes are very crucial in all reactions in our body for metabolism and catabolism.
Q: in cells, an enzyme catalyzes the reaction ab → a + b. it was isolated, however, as an enzyme that…
A: Enzyme catalysis is the use of a biological molecule called a "enzyme" to speed up a process. The…
Q: Consider the Michaelis-Menten enzymes below and answer the following questions. Enzyme Km (M) | Keat…
A: Enzymes are protein molecules that increase the rate of reaction by decreasing the activation…
Q: Enzyme Activity Enzyme A Enzyme B Enzyme C 2 7 pH a. Identify the above-mentioned enzymes. i. Enzyme…
A: Enzymes are the proteins which are the biological catalysts and enhances the natural processes…
Q: This covers how temperature and pH affect the rate of enzyme catalyzed reactions. 1. Consider the…
A: Many metabolic pathways exist in the human body to maintain equilibrium. Inside the cell, metabolic…
Q: Figure #1 and #2 show the results collected from a fun enzyme activity lab that did NOT involve the…
A: Introduction Enzymes are known as biocatalyst. Chemically enzymes are protein. It increases the…
Q: An enzyme catalyzes the reaction MN. The enzyme is present at a concentration of 0.0000000022 M, and…
A: Enzyme catalyzed reaction involves the binding of enzyme to substrate leading to the formation of…
Q: The enzyme becomes denatured if the temperature is compared to its optimal temperature: O a. Too low…
A: Enzymes are the biomolecules that act as a catalyst in the biochemical reaction and can increase the…
Q: 20 40 60 C Temperature 5. What is the optimal temperature for the enzyme in graph C? I Where does…
A: Note: We are authorized to answer one question at a time since you have not mentioned which question…
Q: What is the minimum and maximum pH in which Enzyme X will work? What is the optimal (best) pH? Rate…
A: The enzymes are proteinaceous molecules that helps in accelerating the rate of the reaction that is…
Q: You have a reaction that takes 8 hours to reach completion at 20 degrees celcius. What is the likely…
A: The chemical reactions are of two kinds: Exothermic and endothermic reactions. Endothermic reactions…
Q: Assume you have a solution of reactions with an enzyme. You decided to apply heat to further…
A: All enzymes are made up of proteins. However, not all proteins function as enzymes. Enzymes are…
Q: what happens if an enzyme is not made correctly?
A: “Since you have asked multiple question, we will solve the first question for you. If you want any…
Q: How does changing the concentration of an enzyme affect the rate of decomposition of a substrate?…
A: Introduction: Enzymes are proteins that act as a catalyst which means they increase the rate of…
Q: Some cool enzyme (CE) catalyzes the conversion of compound F to compound A. Which of the following…
A: The option C) CE decreases the energy of the transition state between compounds F and A. ( Detailed…
Q: When is an enzyme able to work properly
A: Enzymes are the biological catalysts that speed up the metabolic reactions that occur in the body.…
Q: What is the percent change for the enzyme active at 40 C compared to 25 C* 220 200 180 160 e…
A: Enzymes are proteins that act as a catalyst to speed up chemical reactions. A catalyst is a…
Q: Which of the following statement/s is/are TRUE of enzymes? 1. They increase the rate of reaction by…
A: Answer is option c.
Q: 1. The concentration of substrate X is high. What happens to the rate of the enzyme-catalyzed…
A: Enzymes are the proteinaceous substances that are capable of catalyzing chemical reactions of…
Q: Enzyme specificity us given by :- а) Km b) Vmax c) both d) none
A: Specificity of an enzyme is a property that describes enzyme's choice for its particular substrate .
Step by step
Solved in 2 steps
- Consider the following free energy diagram for an uncatalyzed and enzyme-catalyzed reaction. Select all the statements that are true. Without enzyme With enzyme A+B Time AB O a. The rate of the enzyme catalyzed reaction is faster than the uncatalyzed reaction O b. The change in free energy for the reaction is greater in the catalyzed reaction, compared to the uncatalyzed reaction O c. The enzyme stabilizes the transition state for the reaction Od. The reaction is exergonic е. The reaction is now spontaneous due to the addition of enzyme Released EnergyExplain the biochemical basis of how temperature and pH affect enzyme activity. What are the changes in the enzymes that these physical factors cause?L(24 points) Explain How is the Michaelis constant defined. and what does a low or high value for Km tell you? What is the difference between the velocity and initial velocity of an enzyme reaction? What determines the efficiency of an enzyme reaction, and what terms are used to describe it? 2. (50 points) About how to obtain kinetic data experimentally Lisa decides to obtain values for the Km and Vmax of an enzyme she has just isolated from liver cells (it is now pure), using a Michaelis Menten plot. Describe in detail what kinds of measurements she would have to make, and what she would need to plot on graphs in order to estimnte the values for Km and Vmax. (Show the kinds of graphs she would have to plot, and how these will allow her to estimate Km and Vmax.) Describe how she would be able to obtain Vmax experimentally and from the Michaelis Menten plot - what conditions are needed and what would be measured). Also, describe what she would have to do to obtain the turnover number of…
- When biological enzymes are heated, they lose their catalytic activity. The change that occurs is an ENDOTHERMIC and SPONTANEOUS process. Based on the given information, what is the sign of Δ˚G? Based on the given information, what is the sign of Δ˚H? Is the structure of the original enzyme MORE or LESS ordered than the new form? Explain your answer.The milieu wherein enzymes occur in vivo is dramatically different from in vitro experiments, which is often used to investigate enzyme. Give an overview of this as well as the nature of enzyme reactions in vivo. Name, in addition, the factors affecting the concentration of enzymes in vivo.Several factors contribute to enzyme catalysis. What arethey? Briefly explain the effect of each.
- An enzymes catalyzed reaction is studied in the presence and absence of an inhibitor. The following data was obtained in the image provided. Plot 1/[S] as abscissa and 1/V as ordinate for both catalyzed reactions and reaction with inhibitor. Use the same graph for both plots Michaelis–Menten kinetics Lineweaver–Burk plot Calculate the following: Km of enzyme in the reaction without inhibitor Km' of the enzyme in the reation with inhibitor Vmax of the uninhibited reaction Vmax of the inhibited reaction What kind of inhibitor was added to the enzyme catalyzed reaction? Explain your answer in terms of changes in Km and Vmax.The steady-state kinetics of an enzyme are studied in the absence and presence of an inhibitor (inhibitor A). The initial rate is given as a function of substrate concentration in the following table: v[(mmol/L)min- [S] (mmol/L) No inhibitor Inhibitor A 1.25 1.72 0.98 1.67 2.04 1.17 2.50 2.63 1.47 5.00 3.33 1.96 10.00 4.17 2.38 (a) What kind of inhibition (competitive, uncompetitive, or mixed) is involved? (b) Determine Vmax and Ky in the absence and presence of inhibitor.Enzyme X exhibits maximum activity at pH = 6.3. X shows a fairly sharp decrease in its activity when the pH goes much lower than 5.8. One likely interpretation of this pH activity is that: a Glu residue on the enzyme is involved in the reaction. a Tyr residue on the enzyme is involved in the reaction. a His residue on the enzyme is involved in the reaction the enzyme uses NADH has a cofactor. the enzyme uses coenzyme A has a cofactor.
- Given the following information, calculate the catalytic efficiency of the enzyme. Step by step please [S] = 100 mM k1 = 10 sec-1 k2 = 3000 sec-1 k-1 = 20 sec-1 [E]T = 1 \muμMwhen saturated with substrate, an enzyme has a maximum initial rate of 110mumoles of substrate converted to product per second. At a substrate concentration of 100mu M, the same enzyme converts substrate to product at a rate of 0.010mmoles/ sec. Assuming that Michaelis - Menten kinetics are followed, calculate the reaction rate when substrate concentration is 2x10^-3M.. The optimal conditions for salivary lysozyme (hydrolyzing glycoproteins of bacterial wall) are 37 C - temperature and pH is 5.2. Explain the decrease in this enzyme activity if the temperature will rise up to 60 °C and pH will be changed to 8.0. To answer the question: a) draw the graph of the velocity dependency on temperature and pH; b) calculate the relative enzyme activity if 10 mg of lysozyme catalyzes the formation of 5 uM of the product per 2 minutes. Concidor NH3: 5.