. What factors can affect a solute's Rf value in thin-layer chromatography? 2. Why is it necessary to follow these steps when conducting the experiment? -No contact with the TLC plate should be made with bare hands. -Spots should have a diameter of no more than 1 mm. -Allow sufficient solvent to saturate the chromatography chamber

EBK A SMALL SCALE APPROACH TO ORGANIC L
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ISBN:9781305446021
Author:Lampman
Publisher:Lampman
Chapter89: Thin-layer Chromatography
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1. What factors can affect a solute's Rf value in thin-layer chromatography?
2. Why is it necessary to follow these steps when conducting the experiment?

-No contact with the TLC plate should be made with bare hands.
-Spots should have a diameter of no more than 1 mm.
-Allow sufficient solvent to saturate the chromatography chamber.

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