Abstract: During photosynthesis plants take light energy and turn it into chemical energy. The purpose of the study was to test the effect of various lighting conditions on the rate of photosynthesis. In this experiment the rate of photosynthesis is measured by timing how long it takes photosynthesis to occur in ten leaf disks that are in a solution of carbon dioxide. The prediction for this experiment was that if a plant receives more light, then it will have a higher rate of photosynthesis. The data supports the hypothesis, because the rate of photosynthesis is higher in direct sunlight than in the shade. This experiment untimely lead to the conclusions that light and carbon dioxide are necessary for photosynthesis to occur. …show more content…
This light is then converted into a chemical energy by the chlorophyll and other pigments within the plant. This energy is used for the process of photosynthesis. During photosynthesis, water molecules split apart, releasing electrons and hydrogen ions and producing oxygen gas. These electrons and hydrogen ions are then used to generate ATP and NADPH. Both ATP and NADPH are also used in the light independent reaction, which is the second part of photosynthesis. This reaction occurs in the stroma of the chloroplasts. In contrast to the light dependent reaction, this type of reaction can occur without light. During the light independent reactions of photosynthesis ATP and NADPH are required to form glucose. Adenosine triphosphate, often called ATP, is used to construct organic molecules from carbon dioxide and water. Nicotinamide adenine dinucleotide phosphate, often called NADPH, reduces carbon dioxide and produces monosaccharides for use by the plant. Materials & Methods: Prepared solution containing 1.5 sodium bicarbonate in 300 ml water and 2 drops liquid soap Large plastic syringe 20-65 ml with no needle #3 cork borer with plungers Spinach Leaves Two plastic cups One glass beaker Timer Light source Paper Towels Cover: Cup covered by foil Mouse pad for cutting surface This experiment was performed using the procedure from the Photosynthesis in Leaf Disks Lab. First using a #3
Photosynthesis is the conversion of light energy to chemical energy into sugars. It is the process in plants that uses carbon dioxide, water, and sunlight from its surroundings and releases oxygen as a byproduct (6H2O+6CO2+light energy -> C6H12O6+6O2). Photosynthesis is required for plants because they are autotrophs, organisms that make their own food. Plants require a specific environment that is ideal to them to be able to carry out the process. Environmental conditions can either increase or decrease the rate of photosynthesis. Things like colors of light, pH, and temperature can all affect the rate of photosynthesis in plants.
The purpose of this experiment was to investigate the effects of light intensity on the rate of photosynthesis in a Moneywort plant. By observing the plant in distilled water mixed with sodium bicarbonate, different light bulbs were targeted onto the plant. The measurement of the amount of bubbles present on the plant during the trial of the experiment enabled us to identify the comparisons between the activity of the light and the process of photosynthesis.
Virtual Lab #5- “Which Colors of the Light Spectrum are Most Important for Plant Growth?
The amount of leaves that floated What problem/question did you answer in the experiment? Does light and dark change how photosynthesis works? 2) Why was detergent added to the solution? To take off the waxy covering on the leaf 3) Why was sodium bicarbonate added to the solution? CO2 source for
14. At this point, a vacuum is to be created within the syringe to draw the air out of the tissue of the leaf disks. After this step, the experiment is really quite simple.
Photosynthesis is a the process in which plants and other organisms to turn light into energy. To see if this is true I will test to see if spinach leaves discs can be effected by photosynthesis. I predicted that more disc would float in the light than in the dark. After writing down my hypothesis, I started the procedure. I cut up the spinach leaves into the disc and put cold water in the three petri dishes so the leaves would float in the water.
Plants occur around the world in a wide variety of environments, but how does the environment affect photosynthesis rate? Temperature, light intensity, water supply, and the amount of carbon dioxide are all factors that contribute photosynthesis rate. For the lab on photosynthesis, our group tested the Anacharis Bunch plant, which is an aquatic plant that needs moderate light in order for photosynthesis to occur (“Anacharis (Egeria densa)”). For photosynthesis to take place, energy from the sun is required. When sunlight strikes a plant, the stoma opens and carbon dioxide along with energy from the sun are absorbed into the chlorophyll as well as water which is transported up through the leaves to the chloroplasts where the chlorophyll are located. The chlorophyll then uses carbon dioxide, water, and the sun’s energy to produce to sugars such as glucose. After the glucose is produced, the energy that is stored in the glucose is then used to form ATP, which is carried throughout the plant and into the chloroplast where the energy is used for photosynthesis and other cellular functions. As a byproduct of synthesizing, a plant releases oxygen and water into the air. Then the cycle of photosynthesis which is made up of two parts, the Light Reactions and the Calvin Cycle, start all over again (“A Primer on Photosynthesis”). To figure out the rate at which the Anacharis Bunch plant photosynthesizes under different conditions, my group and I decided to test the
During this experiment the scientist tested this question “What combination of light intensity, light color, and CO2 level would produce the highest amount of photosynthesis?” The scientist believed the highest amount of CO2 level and light level along with each using white light would produce the highest amount of photosynthesis possible. The amount of oxygen bubbles we got ranged from 4 to 28. However when we put the results from each test together, we only got 26 bubbles. The scientist believes with more testing of each combination, we could find a better ratio. The data unfortunately did not support the scientist’s hypothesis.
The main source of energy in photosynthesis is light energy, which is converted to glucose sugar, and later converted into ATP to provide energy to the cells. In the first phase, photons of sunlight hit the thylakoid membrane, exciting chloroplast molecules, inducing the transport of the electrons extracted from water splitting to form oxygen, down an electron transport chain, much like the one in cellular respiration. In this electron transport chain, the final electron acceptor is NADP+, which is reduced to NADPH to be used later in the Calvin cycle. Much like in cellular respiration, a proton gradient builds up within the thylakoid, and protons are passively transported from the thylakoid lumen to the chloroplast stroma through the enzyme, ATP synthase which phosphorylates ADP to make ATP. This type of chemiosmosis of protons to create ATP energy is uniquely called photophosphorylation. In photosynthesis, carbon dioxide is taken up from the atmosphere from the plants’ stomata, ultimately to create glucose molecules. The oxygen released from water splitting by photosystem II is crucial for almost all life. Overall, the process of photosynthesis is anabolic, as it builds up a large molecule, glucose from less complex smaller molecules, while requiring energy to do so.
In a light- dependent reaction there must be light in order for it to undergo its process. This process is very fast and is used to split water and release oxygen, electrons, and protons. The light-independent reactions are biochemical reactions and convert carbon dioxide to sugar (Rathbone, 2016). This study was performed in order to help know the main fundamentals of photosynthesis in hands on experiments with spinach leaves.
Claim: What affects the rate of photosynthesis is the amount and type of light source that is provided. If you were to have more light than the process of photosynthesis will occur faster than if the light source was not a lot and dim.
Above the ideal temperature the rate begins to decrease, until the photosynthesis finally stops. as the temperature increases, The enzymes get more energy when the temperature increases so the rate of photosynthesis increases. If it gets too hot the enzymes begin to lose their shape so they are unable to function properly and the rate of photosynthesis decreases. This explains why in the hot temperature the oxygen percentage was the lowest because the temperature surpassed the ideal temperature for photosynthesis, which is between 0 degrees celsius and 35 degrees celsius. Although this experiment rejects our hypothesis and our background research, we believe we had an error in producing our experiment. We believe that in the cold temperature, the temperature was not consistent since the ice melted and in the cooler the plant received more light due to the white walls surrounding the plant that reflected the light.That is the reason our data does not match the principles of photosynthesis. To keep the temperature consistent, we suggest putting the cold temperature plant in a freezer which is about -17.77°Celsius without opening the freezer for the 24 hours the plant will be in the
As the table lamp is positioned nearest to the pond weed, it shows highest rate since higher light intensity means more photons are striking at the surface of leaves. When the light intensity rose from low to high, it will cause the rate of photosynthesis to increase due to more light energy presents to operate the reactions of photosynthesis. Generally, when the intensity of light is high, a greater rate of photosynthesis will be achieved. However, this rate has a limit, and once that limit is reached the rate cannot be increased to past that limit. It is because the oxygen gas will start to compete for RuBP carboxylase with carbon dioxide at a certain light intensity, whereas the more oxygen gas binds to the enzyme, the less glucose is produced,
Photosynthesis has a two-stage performance before plants produce the two products they are known to produce. These stages are Photosystem I and II. Photosystem II is dependant on light reactions for energy which causes the electrons to be react and be transferred to Photosystem II. The electrons are transported through the Photosystem II electron transport system, however some energy is used to drive ATP synthesis. Meanwhile, light is being absorbed by the Photosystem I, which causes the electrons to react. This process sends the electrons to the Photosystem I transport system where some energy is released as electrons travel through the electron transport system and is captured as NADPH. When this process is completed oxygen is released from the plant and glucose has been
To metabolic pathways involved in photosynthesis are light reaction and dark reaction. The first stage of the photosynthetic system is the light-dependent reaction, which converts solar energy into chemical energy. Light absorbed by chlorophyll or other photosynthetic pigments is used to drive a transfer of electrons and hydrogen from water to and acceptor called NADP , reducing it to the form of NADPH by adding a pair of electrons and a single proton. The water or some other donor molecule is split in the process. The light reaction also generates ADP, a process called photophosphorylation. ATP is a versatile source of chemical energy used in most biological processes. The light reaction produces no carbohydrates such as sugars.