For this experiment, my group had several leaves of spinach and hole punched 40 disks out of the spinach leaf. In order to avoid any bias results, we made sure to avoid hole punching any veins in the spinach. After that we filled 4 cups each with 100 milliliters of carbon water. Each cup would have a different amount of salinity, the first cup would be the control group which would have no salt in it. The second cup would have ¼ of a teaspoon of salt in it, the third cup would have ½ of a teaspoon of salt in it, and the fourth cup would have ¾ of a teaspoon of salt in it. Next, my group would take 10 leaf disks and put them into a clean 10-ml syringe by removing the plunger. My group made sure that all 10 disks were near the tip of the syringe before we re-inserted the plunger to avoid injuring the disks. …show more content…
I would than hold the syringe tip upward and expel the air by depressing the plunger carefully. I would than seal the syringe by covering the tip of the syringe with my index finger in order to create a vacuum. I would than pull back on the plunger and then push in to cause the leaf disks to sink. I made sure to let go of my index finger to observe if my groups leaves sunk, if not then I would push and pull again until the leaves had completely sunk. Sometimes there would be leaves that were stuck and I would have to tap on the syringe in order to get it to sink. After that, place the 10 sunken leaf disks into the 4 cups that we had prepared earlier. Repeat the syringe portion, each time with 10 leaves and place them into a different prepared cup. After all that has been prepared, place a lamp 19 centimeters high and turn it
In this experiment, 4 grams of peeled turnip was used to prepare the enzyme extract opposed to the 1 gram of turnip suggested by Fundamentals of Life Science. Along with the change to the amount of turnip used, the amount of 0.1M phosphate buffer used to prepare the enzyme extract was changed from 50mL to 30mL. The affect of temperature on enzyme activity was not
Add three seeds to the potting mix and cover seeds with little remaining potting mix. After the addition of the potting mix, use a dropper filled with water and water each cell until water drips from the wick. Then place the quads on a watering tray under the fluorescent light bank. Each cell should have an equal distance from the light bank. Quads should be three inches below the fluorescent light; the light should also be left on all day. Make sure all wicks are in contact with the mat that sits on the watering tray. Also watch out for the watering system regularly throughout the experiment. After four to five days record plants in the quads, giving their phenotypes in a table for each cell removed all but the strongest plant.
I filled three clear cups, the first one with dH2O, the second with 0.1% NaHCO3 solution (equal parts 0.2% NaHCO3 and dH2O), and the third with 0.2% NaHCO3 solution. The control of the experiment is the cup with dH2O. The independent variable is time and the dependent variable is the number of disks floating in the solution. We separated the 30 disks into three groups of 10, placed them in syringes filled with a corresponding solution (either dH2O, 0.1% NaHCO3, or 0.2% NaHCO3), and removed all air from the syringe. This causes photosynthesis to stop in the disks, which then causes the disks to not float any longer. The three groups of disks were placed in each cup filled with 100mL corresponding to the solution, then placed under a light source and started a timer. For each minute in 15 minutes, data regarding the number of disks that floated to the top of each
In generalization, there are a multitude of factors that could potentially influence the germination of a radish seed. This lab thoroughly exhibits the effect of water amounts on the germination of a radish seed. There is indeed an in-depth science behind the projected results, and overall of the effects water has on the germination of radish seeds, and the growth of plants in particular. Radishes themselves are moisture-loving plants; therefore, it is significant that they receive an adequate amount of water, allowing the soil to be moist, but not overly saturated (Biology Coach, 2015). In general, water is significant for the health of a plant in the way it transports important nutrients throughout the plant. From this point, nutrients are drawn from the soil and used by the plant. Seed germination itself is defined as the process where the seed sprout for growing, and future development into a plant. In order to germinate, the seed must have its essential needs met until it is capable of doing so: water, temperature, and sun. Therefore, during its early stages of growth, the seed will rely upon the food supplies stores within it, until it is large enough for its own leaves to begin making food through photosynthesis (Biology of Plants, 2016). Initially, the process of germination begins with the absorption of water y there seed, therefore, this absorption of water then activates an enzyme that increases
A plant's growth ability is dependent on its ability to acquire the resources it needs to survive. Competition such as interspecific and intraspecific, limiting resources, and population density affect the fitness level of a plant. This experiment was conducted in order to test the capability of collards and radishes to grow in manipulated densities under interspecific and intraspecific competition. I hypothesized that both collard and radish plants will grow more efficiently in single species pots under low-density conditions. I also hypothesized that in the mixed species plots the radishes will be more fit to survive and grow better than the collard plants in both the high and low-density pots. Both high and low density and single and mixed species plots were planted and results were observed. There was a significant
During the experiment, my partner and I received 0 leaves floating in the 0% concentration which was expected. But we also gathered the data that 0 leaves floated for 0.2% the first time we tried which isn’t expected. The second trial we changed the distance of the light and brought it closer and 4 of our 10 leaves floated in the 15-minute span.
51) A botanist wanted to see if a new strain of corn could germinate in soil that was too salty for regular corn. She conducted a study on the germination success of seeds from the new strain that were exposed to various levels of salty soil, from zero to normal (100mg/L) to high (200 mg/L) to very high (400 mg/L) to normally lethal (800 mg/L)
Wait, another 3 minutes. While waiting for Plant A to soak, proceed to put Plant B (the leaf exposed to no air through soda lime) into the same beaker of boiling water. Once time is up for Plant A, rinse the leaf with water and place leaf off to the side. Next, grab tongs and place Plant B directly into the beaker with alcohol solution (Making sure to grab new alcohol into the beaker and not using the same solution from Plant A). Wait, another 3- 5 minutes. Once time is up place Plant B, using tongs, into the petri dish and completely cover the leaf in iodine solution. Wait 3 minutes. Then rinse off the leaf and compare the coloration with Plant A. Record
Get a 10.0 mL pipet and suction bulb then use deionized water to rinse the pipet. Then use the rinsed pipet to add 10.0 mL of deionized water to the flasks weighed previously.
Rinse the penny skin with distilled water and set it aside for a moment. Empty the solution in the beaker down the sinking with running water. Rinse out the beaker several times and gill it half full with distilled water.
The next step included choosing appropriate Hydrilla and placing in the funnel in such a way that the uprooted part faces upwards.
Without touching outer sides or bottom of ampule, insert needle into medication ampule, and draw up the appropriate amount of solution (0.3-0.5mg).
The experiments were performed in the science lab 1.226 at the University of Texas Rio Grande Valley, Edinburg on October 2, 2017. The experiments were performed in a two-day process due to lack of time. Instructions were given by our TA on where to find the substances (guaiacol under the fume hood, turnip extract, peroxide, and distilled water were placed on our lab tables in dropper bottles, along with the spectrophotometer) and were told to get started. In activity 1 we will be testing 3 concentrations of an enzyme (0.5 ml, 1.0 ml, and 2.0 ml of turnip extract). To quantify the rate of reaction in turnips, guaiacol will be used as the color reagent. Guaiacol is oxidized when it encounters peroxide, allowing light at 470 nm to be absorbed and allowing us to measure the absorbance. In the first activity from experiment day 1, three test tubes were obtained and two clean cuvettes from our lab TA, and placed in a test tube rack on our lab tables. We used one of the test tubes to make the control, another to make the substrate and the last one to make the enzyme. We did this process 3 times to test the effects of the low enzyme concentration, medium enzyme concentration, and high enzyme concentration on the enzyme reaction rate. For the low enzyme concentration, on the control test tube we added 1.0 ml of guaiacol, 0.5 ml turnip extract, 0 ml of peroxide and 8.5 ml of distilled water, getting a total volume of 10 ml in the test tube. For the low enzyme concentration, on the
In order to make the experiment better, the apple skin could have sat in the salt water solution for a longer period of time to insure the salt water is actually getting into the apple skin. The amount of trials also could have been increased to insure
1. Lab reports are to be computer-generated and double-spaced. All sections of the report must