Make Five Standard Solutions

The mixture was cooled down to room temperature and transferred to a separatory funnel where it was washed with four portions of 50 mL of water.

The solution was heated till boiling and vigorously stirred. 10mL of 10% barium chloride solution was added drop wise from a measuring cylinder.

Pick one of the test tubes that have 0.2 ml of silver nitrate. Then pipet 2 ml of the buffer/extract/silver nitrate mix into an empty cuvette. Place the cuvette into the spectrometer. Follow the directions from the start of the lab to calibrate the spectrometer again. (Note: You can skip

Introduction: Substances can be created and can go through a chemical change. When a substance is produced this is a reaction or chemical change this is also called the product. If a substance is the reason why the experiment this is called a reactant. Whether it is a product or a reactant it depends on what happens in the chemical reaction. There is many types of chemical reactions.

Using a Pasteur pipette, add 1.0 milliliter (mL) of hot water drop-by-drop into the reaction tube to reach complete solubility of the solute

Obtain two 10.0 mL graduated cylinders, a clean cuvette, and a small beaker. In one fill with 10.0 mL of sodium hydroxide and in the other fill with 10.0 mL of crystal violet solution. Pour both of the 10.0 mL graduated cylinders into the small beaker simultaneously. Manually swirl the solution for a few seconds. immediately fill the cuvette ¾ full of the solution, discard, then fill ¾ full again and place in the calorimeter, making sure to wipe the cuvette with a lint free tissue (Barrett and Stephenson). Click the COLLECT button in the Logger Pro software followed by the KEEP button after five seconds has elapsed. For 20 minutes continue to press the KEEP button in 20 second intervals. After 20 minutes, remove the cuvette and dispose the contents down the sink along with the remaining solution in the

important step before checking the absorbance value is to tare or blank the cuvette first

was placed in a clean dry 100 mL beaker. The initial pH of DI water before the addition

Phosphate buffer, 0.1 M, pH 7.4 at 4ºC, containing 20% v/v glycerol 0.9M sodium dithionite (note: solution should be kept on ice) 11.94 mM NADH (note: solution should

Add 2-3 grams of each compound to the test tube with the correct label on it, then measure and record the mass of each test tube and the compound.

To do so, we used the cuvette 0, also known as the blank, and the cuvette 5, the pure substance. We calibrated our spectrophotometer by setting the λ (wavelength) to 383 nm and then placing the blank in. Afterwards, we set the transmittance to 100%. Then, we removed the blank cuvette and placed the cuvette 5 in. After recording the %T and absorbance, we repeated the process but with a different wavelength. We repeated with wavelengths ranging from 383 to 700, recording the %T and absorbance each time.

We were given 0.1% (w/v) stock solution of isoprenaline hydrochloride in water, we prepared 50 ml of 0.01% w/v isoprenaline hydrochloride this was done by calculating the dilution factor first which was 10 this was calculated by:0.1/0. 01=10.After calculating the dilution factor we worked out how much of 0.1% (w/v) stock solution isoprenaline hydrochloride was needed and how much double distilled water was needed.5 ml of 0.1% (w/v) stock

Standard solution of Trazodone hydrochloride raw material at 5 ppm: Transfer 0.5 mL of 100 ppm Trazodone hydrochloride stock solution to a 10 mL volumetric flask. Complete to volume mark with the diluting solvent and invert flask at least three times to ensure a proper mix.

In order to perform this experiment, proficiency with using spectrometers, burettes, volumetric flasks, weighing by difference, and making a hot-water bath are essential1.

Pipetted 325 μl of tube 1 solution into tube 2, and added 325 μl of PBS into tube 2. Mixed the solution by pipetting.