October 17, 18, and 19, samples were collected from multiple sites along the BSR. The class was split into groups, and samples were collected from seven separate locations along the river and WWTP. There was also a sample collected by the S which is located between sites four and five. For each of these sites, there were ten groups from other labs that also collected a sample from the BSR. At site two of the river, the location included multiple sources of possible contamination. A drainage site was located 200 yards upstream, along with a small PVC drainage pipe next to the collection site. Not only was there drainage running into the river, the site was under a bridge, and contained other trash scattered throughout the area. The …show more content…
Once these steps were completed, the cuvette was placed into the spectrophotometer to test for phosphate, nitrate, ammonia, and turbidity. Inside the spectrophotometer, a light was shot through the sample to detect the contaminant. The first ever spectrophotometer was only developed to calculate pH levels and never used wavelength to calculate the abundance of chemicals within a sample. After many years of developing the spectrophotometer, a glass prism was installed. This allowed the spectrophotometer to use light wavelength to calculate the concentration of chemicals within a sample. The light was able to read the preferred contaminant because a reagent attached to each molecule, and the light was reflected back instead of being shot through the sample. This gave the concentration of each contaminant in the sample. In each machine, there was a different light wavelength programmed to shine through the sample to detect the different contaminants. This was done to get an accurate measurement of phosphate, nitrate, and ammonia within the sample. Turbidity was also measured using the spectrophotometer, but there was no reagent added. The turbidity was calculated by testing distilled water first and comparing it to the turbidity of the BSR water. Microscopes were used to look at the different invertebrate organisms within the BSR. There were two stations that included two
The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in
My unknown organism #6 is Morganella morganii, which is a gram-negative bacillus rods commonly found in the environment and also in the intestinal tracts of humans, mammals, and reptiles as a normal flora. (3, 5) This bacterium Morganella morganii, was first discovered in the 1906 by a British bacteriologist named H. de R. Morgan. (2) Despite its wide distribution, it is an uncommon cause of community-acquired infection and is most often encountered inpostoperative and other nosocomial settings. (2, 3) Morganella morganii infections respond well to appropriate antibiotic therapy; however, its
An association between enzyme production, gene copy number, and gene evolution was explored by conducting analysis of the salivary amylase enzyme, AMY1A gene copy number, and the ancestral starch consumption in Homo Sapiens (Tracey 2017, p.22). It was hypothesized that the relative amount of starch consumption was very high for my personal ancestral diet, thus my AMY1 diploid gene copy number in my genome and salivary amylase concentration would be significantly higher than the population mean. With a population of 28 subjects (n=28), individual saliva samples were collected and compared to a calibration curve to determine the approximate amylase concentration by analyzing absorbance values. Individual samples of buccal cheek cells were
The experiments conducted for this lab report focused on water contamination and filtration. Experiment 1 was effects of groundwater contamination. Oil, vinegar, and laundry detergent were added to clean water with no means of filtration. The clean water was found to be contaminated. A filtration system consisting of cheesecloth and 60 ml of soil was created and the contaminated samples were filtered through it. The soil and cheese cloth did not affectively filter the contaminants. Experiment 2 focused on
2. (5 pts) List and explain the names and affiliations of the various characters/stakeholders in this story – I’m looking for us to use the story to map out the complexities that are generally associated with solving public health puzzles – the stakeholders you list and explain here should apply to many of the cases we consider going forward.
The investigation is showing how enzymes work inside a mammal's stomach. Rennin is the enzyme found in young mammals and has more effect
An unknown was given to our group from the professor. The unknown was in nutrient broth, the group received unknown number 3. And the task was to identify the unknown and try to make an educated guess, and identify the unknown #3.
Stover, C. K., Pham, X. Q., Erwin, A. L. Mizoguchi, S. D., Warrener, P. Hickey, M. J., Brickman, F.S. LOlson, M. V. (2000) Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature: International journal of science. Nature 406: 959-964. Retrieved from https://www.nature.com/articles/35023079#main
Background: High blood glucose fluctuation (BGF) contributes in chronic complications by promoting the dysfunction of vascular endothelium in diabetes. MicroRNA (miR)-34 family is known for its roles in the pathophysiology of diverse diseases including type 2 diabetes. However, the action of BGF on the expression of these miRs in diabetes is unknown. Herein, we aimed to investigate the effects of BGF on the pathogenesis of diabetes and the possible involvement of miR-34 family in vivo.
The main purpose of these experiments was introduce various techniques such as the use of separation, purification and extraction for each organic compound that was obtained. The first experiment was done on a compound which is commonly known as a triglyceride which contains three fatty acids and a glycerol backbone. The next experiment was done one a caffeine which is a hetercylic ring. However, this heterocyclic ring has all oxygens in the place of the carbons. The third eextraction was done on benzoic acid and acentanilide and the last experiment was done on acetic acid to purify an ester.
SKBR3 (breast adenocarcinoma) and BT-474 (breast ductal carcinoma) cells, which are HER2 positive models, were purchased from DMSZ (Braunschweig, Germany). SKBR3 cell line was grown in Dulbecco’s Modified Eagle Medium (DMEM; Gibco/ Invitrogen, Carsbad, CA, USA) and supplemented with 15% fetal bovine serum (F.B.S; Gibco/Invitrogen), 10mg/ml insulin (Sigma Aldrich, USA) and penicillin-streptomycin (100 x solutions, Invitrogen). Moreover, BT-474 cell line was cultured in RMPI-1640 (Gibco/Invitrogen) and supplemented with 20% F.B.S, 10mg/ml insulin and penicillin-streptomycin. At last, MCF10A (breast fibrocystic disease) cell line was cultured in DMEM/Nutrient Mixture F-12 (DMEM/F12;
The size of the particle should be large enough to allow three proteins coupling together onto one particle. Therefore, Dynal® MyOneTM 1-μm carboxyl particle (Invitrogen®) and Merck® EM1-100/40 particle were used as the carrier particles. If positive results were seen, the work would be expanded to particles in other sizes, materials and manufacturers, such as Allrun® PM3-050 or Dynal® M270 particles.
CE3.13 Since I had previous experiences in using the survey software, I recommended the usage of Ekahau site survey. Even though there are different survey tools were available in the market, the reason I selected this tool is that it facilitates WLAN planning at the pre-deployment stage. I advised Mr. Surya, who was the lead systems engineer, to use of this tool for the detection of the coverage holes and co-channel interference zones in the various location of campus. Ekahau wireless adapter was used to analyse the coverage holes and Ekahau site survey software was used to monitor the coverage holes. Heat maps to detect the holes in coverage and analysed the coverage distribution in software tools. Since this project was aimed to cover
Observations of imploding thin-shell capsules have demonstrated an increasing discrepancy between predictions from single fluid hydrodynamic simulations and experimentally measured fusion yield as the Knudsen number (mean-free-path over system size) is increased. (-- removed HTML --) (-- removed HTML --) 1,2 (-- removed HTML --) (-- removed HTML --) The sensitive dependence on the Knudsen number indicates that these discrepancies may be due to a breakdown of single fluid hydrodynamics and thus motivates the need for multifluid (-- removed HTML --) (-- removed HTML --) 3,4 (-- removed HTML --) (-- removed HTML --) or kinetic modeling. (-- removed HTML --) (-- removed HTML --) 5,6 (-- removed HTML --) (-- removed HTML --) Specific
One crucial step in the data acquisition procedure was to make sure that the wind speed and wind direction were in accordance to the target ranges. This will be more tangible if it is pointed out that time histories of pressure coefficient are directly dependent on the velocity measurements and turbulence intensity at reference height in the lab. Therefore, wind speed measurement has to be performed with high resolution devices that can catch little changes in wind characteristics; to do so, in the current study Cobra probe technology was used. More details regarding the high tech instruments that were used for experimental setup are presented as follows.