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Lab Report

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Methods: Due to experimental error in previous labs, we did not obtain the DNA of our original taxa. We then chose to use DNA sequences supplied by the TA from the 2016 and 2017 school year. We picked six fungal taxa obtained from differing parts of English Yew Trees, including its fruit, bark, and leaves. Using the DNA sequences given, we copied the sequence into the BLAST system found on blast.ncbi.gov, making sure to add “fungi” into the organism box and to search for somewhat similar sequences. Once the program obtained the results, we scrolled down to the first result that gave a genus and species name, with an “ITS” in the description. Using the information given in the results, we filled in the information on Table 2, including seq …show more content…

The question asked for this experiment was, “Are there different endophytes on different parts of an English Yew, and are they related?” As seen in Figure 3 and Table 2 different endophytes were found on separate parts of the plant, and they are related. The question as we realized was quite broad because all organisms are related to some level. We were able to discover the organisms with a 0 E-value (meaning there was 0 probability of the strands matching up being chance). There was a variety of fungi found, with two that were the exact same organism. As seen from figure 3, there is a relation between the different endophytes. Outline of Discussion (example of interpretation of results)When studying endophytes, the main thoughts of the members of the group were that the specific parts of the English Yew, ie, the berry or the bark would have endophytes more closely related than to the different parts. Looking at Figure 3, there were two of the same endophyte found on the berry and the leaf of the English Yew. The outgroup found was Pseudonyms, (found on the bark) which is an environmental yeast. This could have been found because of the different stages a plant goes through. An interesting discovery was that the two berry samples taken had completely different endophytes found. Same with the bark and the leaf. It seems as though there are a lot of complexities going on with these different endophytes,

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