Identifying The Unknown Organisms After Performing A Gram Stain

An unknown bacterium was handed out by Dr. Honer. The appropriate tests were prepared and applied. The first procedure that was done was the gram stain. Under a microscope, if the gram stain is purple, the bacterium is gram positive, if the stain is red, it is gram negative. The next test was the fermentation tests for glucose, sucrose and

The presence and variety of microorganisms in water accounts for most of the indicators of water quality. The presence of Coliform is a big indicator of fecal contamination in freshwater sources. Coliform bacteria is an organism that is naturally present in the human digestive tract; these tend to be rare and

There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient, so as to know how it can be treated, to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that I have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium.

There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient, so as to know how it can be treated, to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of unknown bacteria. The identification process can be completed with a series of deferential stains and biochemical tests. Creating a dichotomous key helps to limit the amount of biochemical tests done on an unknown organism and by observation

My unknown organism #11 is Escherichia coli (E. coli). E. coli belongs to the family Enterobacteriaceae. E. coli is Gram-negative, facultative anaerobe, and rod-shaped bacteria (bacillus). [1]

“Diagnosis is based on symptoms and the results of a biopsy of tissue from the small intestine or other

Water-borne human infectious diseases are associated with human or animal faeces. Animals are becoming a worry, globally and they place an enormous trouble on the human population of many countries (Domingo et al. 2007).Bacteria known as Faecal indicator organisms (FIO) were used to indicate the occurrence of faecal contamination. The most common FIO are Escherichia coli and Enterococci. These organisms are ideal to

This study was done to determine unknown microorganisms that were retrieved from pond water in Rocklin, California. If the study involves identifying microorganisms from water, various amount of test are done to find what type of bacterias are in water. The most common bacteria in the genera Eschericha, Pseudomonas, Acinteobacter, and Aeromons are amongst the top bacteria found in water. As a result, one or more of these microorganism will be found in the pond water collected. It is important to specify one of these bacteria in the water sample as it is potentially harmful. Some strains of Eschericha Coli are known to cause “serve gastrointestinal infection, dysentry and even death in susceptible individuals”(Wilson, 2015). Most importantly, it is important to find these species that can cause danger to humans. So, they can be treated accordantly with the right antibiotics if they presented any of the side effects.

The API 20E is used for the identification of gram-negative bacteria and specially of the family Enterobacteriaceae. This system utilizes a plastic compartmentalized in 20 spaces. Those compartments are a cupule and a small tube that contains a specific dehydrated substance. After you inoculated each compartment with an unknown bacteria, a reaction will occur in 18-24hours. The results were organized in a Reading Table and given a number based on the results. With this number it is possible to identify the bacteria.

The identification of an unknown bacterium was done by streaking for isolation on a Trypticase Soy Agar (TSA) plate then by following the Gram stain protocol described in “Stain Protocols” by Smith (1). both unknown bacteria were labeled as letter A but then given nickname to describe their morphology on the TSA plate. The specific steps of the gram stain and other test that were used and further information was drawn from the documents “lab 1 microscope, inoculation, isolation” that were provided by the instructor (2) “A photographic Atlas for the Microbiology Laboratory” (3) are referenced below. The first step done was to streak the mixed unknown for isolation on a TSA plate, using proper sterilization and inoculation techniques to

The result of this experiment was prevented growth of any microorganism in nutrient agar out of aseptic technique and that what we expected and we achieved to our goal of this lab. Another section of this lab, we using another technique known gram staining. Gram staining is a common technique used to differentiate two large groups of bacteria based on their different cell wall constituents4. This technique helps to identify among Gram positive and Gram negative groups by coloring these cells pink or violet. Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan in their cell walls, which retains the crystal violet these cells are stained with.

coli bacteria can potentially cause a wide range of diseases such as: typhoid fever, dysentery, cholera, amebiasis, hepatitis A., and giardiasis (Microbiology: Laboratory Theory and Application, 2010). However, specifically testing for each of these potential pathogens is not practical. It is far more practical to test solely for fecal contamination by using microflora common to the intestines of warm bodied animals. In the case of this lab our water sample was from a Maine rooftop and is not of interest to our community. This water is not meant to be used for recreational use and is not expected by our community to be potable. Our initial hypothesis for this sample was that the sample would test positive for fecal coliform, so the sample was tested for the presence E. coli and other coliforms. E. coli is a gram-negative rod-shaped facultative anaerobe, and is a member of Enterobacteriaceae (Microbiology: Laboratory Theory and Application, 2010). E. coli is a practical candidate for laboratory testing because it can survive outside the body, is abundantly found in the feces of warm bodied animals, and can be easily

Having clean water is a very crucial aspect of health. Many people don’t have access to clean water, this can be life threatening. There are lethal bacterial infections that are present in water. There are many different diseases that can acquire by drinking contaminated water like: cholera, typhoid fever, and dysentery. The biggest concern and issue is the ingestion of water that is contaminated with fecal matter (1). It has been discovered that the main source of bacteria is this fecal contamination. Fecal matter can enter water sources by runoff from farms or any area that contains many animals. This contamination can also come from insects, rats, or other small animals entering wells or water sources (2).

Water sources are susceptible to bacterial contamination from animal and human pollution sources. Pollution of waterways has significant implications on human health, especially from a pathogen perspective. Strategies are used to minimize pollution and the impact on human health. However, these methods can be limited

Micro-organisms are almost omnipresent. Most microbes play a significant role in the nature where certain pathogenic microbes can contaminate food and water, and cause a variety of infectious diseases in living organisms (Invitski,et.al.1999). As of 2015 WHO factsheet, 663 million people rely on unimproved water sources, including 159 million dependent on surface water and at least 1.8 billion people use a drinking-water source contaminated with faeces. Waterborne pathogens cause 10–20 million deaths and 200 million non-fatal infections each year (Leonard et al., 2003). Detection of different groups of pathogens with precision and rapidity is a great