Enzyme Lab

Are enzymes and temperature related? Collisions between all molecules increase as temperature increases. This is true because as temperature increases, so

Step 1: Label the test tubes 1, 2, 3 and 4, where test tube 1 represents the sample placed on the counter at room temperature, test tube 2 represents the tube placed in the refrigerator, test tube 3 represents the tube placed in the freezer, and test tube 4 represents the sample exposed to boiling water. You will expose catalase to each of these four conditions.

• Fourthly, we kept the temperature at a constant 25°C using a water bath. At low temperatures, an increase in temperature causes an exponential increase in enzyme activity. This is because an increase in temperature provides more kinetic energy for the collisions of enzymes and substrates, so

Temperature controls the speed the enzymes work at. Higher temperatures increase the kinetic energy which increases the chance of collision therefore speeding up the rate of

[An active site can be altered by a non-competitive enzyme which encircles the enzyme and alters the shape of the active site which could be very dangerous.]

Enzymes are biological catalysts, which speed up the rate of reaction without being used up during the reaction, which take place in living organisms. They do this by lowering the activation energy. The activation energy is the energy needed to start the reaction.

These results show how temperature of extreme high, or low affects enzyme activity. The highest rate of enzyme activity occurred at 37 Cº. Anything that was hotter or cold than 37 Cº slowed the reaction rate. As I thought, 100 degrees would denature the enzyme, and that was the case. The data provided shows exactly what temperatures enzymes work best, and worst. The objective was achieved as we discovered the different reaction rates under different temperatures. The results are reliable, as we know enzymes do not work well when under extreme heat or denaturation occurs. What I learned in this experiment was that enzymes don’t work well under cold temperatures because they tend to move slower. My hypothesis did not quite match, because I thought they work best at lower temperatures.

If temperature of the water(enzyme environment) is increased to 35°C, then the enzyme activity will

Enzymes break down molecules in our body faster than they would normally break down without enzymes.

An enzyme is a biological catalyst that speeds up the rate of reaction in certain biological functions. They play a vital role in many aspects of human physiology and are necessary for the functioning of a number of systems, for example in the digestive system to help to break down food. All enzymes have a unique active site that can fit on to a particular molecular arrangement on a target substrate; a substance e.g. carbohydrate, protein, or fat, that the enzyme is designed to breakdown. There are a number of different enzymes in the human body; each type produced specifically to perform a certain role. Enzymes are not themselves destroyed in the reaction to break down a

Five different temperatures of enzyme (spinach extract) (5°C, 20°C, 35°C, 45°C and 65°C) were added to individual measuring cylinders -each filled with 7ml of Hydrogen Peroxide (H202). The height of foam (oxygen + water) produced by the reaction was recorded for each temperature of the catalase after 30 seconds, to find at which degrees the enzyme activity had the fastest reaction rate. The data collected from this experiment suggested that the enzyme extract had the greatest efficiency at 20 °C, and the temperatures greater displayed a decline in rate of reaction.

Enzyme Lab Conclusion In this lab three hypotheses were tested. If the catalase Solution is added then, the H2O2 will be broken down into harmless byproducts/ there will be an increase in temp compared to the control, if the boiled catalase is added to the H2O2, then there will be no significant increase in temp compared to the control, and if the catalase with acid is added to the H2O2, then there will also be no significant temperature increase compared to the control. The first hypothesis of, if the catalase solution is added to the H2O2, then there will be an increase in temp compared to the control, was proven correct. The control(the H2O2 with no catalase added) stayed at a constant temp of 23.5 degrees celsius throughout the 5 minutes

In comparison to the control group, the results showed an incline when temperature was increased and a decline when the temperature was decreased. The rise of oxygen production by increasing temperatures show that the substrate H2O2 is gaining kinetic energy which is making the rate of substrate and enzyme collisions more frequent. Also, it is this gained kinetic energy at extreme temperatures which vibrate the molecules of the enzyme until its hydrogen bonds cannot hold together anymore (Rsc 2004). Most enzymes are made of polypeptides that hold their structure by these hydrogen bonds. The breaking of the bonds permanently changes the globular shape of the enzyme which is known as denaturation.

generally act as a catalase that initially bring about a chemical reaction.” Enzymes play an

To find the effect of temperature on the activity of an enzyme, the experiment deals with the steps as follows. First, 3 mL if pH 7 phosphate buffer was used to fill three different test tubes that were labeled 10, 24, and 50. These three test tubes were set in three different temperature settings. The first test tube was placed in an ice-water bath for ten minutes until it reached a temperature of 2° C or less. The second tube’s temperature setting was at room temperature until a temperature of 21°C was reached. The third tube was placed in a beaker of warm-water until the contents of the beaker reached a temperature setting of 60° C. There were four more test tubes that were included in the procedure. Two of the test tubes contained potato juice were one was put in ice and the other was placed in warm-water. The other two test tubes contained catechol. One test tube was put in ice and the other in warm water. After