Cellular Respiration Lab Report

All cells in the human body require sufficient amount of energy in order to sustain life. Cells get their energy through a process called cellular respiration. In this process cells use glucose in the presence of oxygen as a fuel source to synthesize highly energetic molecules of adenosine triphosphate (ATP). ATP is immediately consumed after its formation, so the process of cellular respiration is constantly ongoing. The starting components, glucose and oxygen are converted into carbon dioxide, water and energy. The process of cellular respiration can be divided into three stages: glycolysis, Krebs cycle (citric acid cycle), and the electron transport chain. At the end of the process a total of 38 ATP molecules are produced. In this experiment,

2. Both the measurements of temperature and volume limit the precision of the data because for temperature, we could only round to the nearest tenth, which limits the amount of sig figs. In addition, because the total volume was only 50mL, there could have been another volume that would have exceeded the optimal ratio of this experiment.

Some knowledge that is needed before performing this lab are as follows: First of all, cellular respiration is the metabolic processes whereby certain organisms obtain energy from organic molecules. This process includes glycolysis, the Krebs cycle, and the Electron Transport Chain. Glycolysis is a process that takes place in te cytosol and it oxidizes glucose into two pyruvate. Glycolysis also makes ATP and NADH. The Krebs Cycle occurs in the mitochondria and this process takes the pyruvate and breaks it down into carbon dioxide. But it also produces 3 CO2, 1 ATP, 1 FADH2, and 4 NADH. The electron transport chain takes place in the inner mitochondrial

With this experiment, I felt there were a number of possible scenarios of error. The first part I notice that could have caused an error in my overall sample was at the start of the experiment I heated my unknown and the flask in a beaker of water that was not boiling yet for several minutes. Once I noticed my mistake I heated the water until boiling temperature recorded the degrees and continued on with my experiment. The next part of my experiment that may have caused error to my overall sample was while I was lowering my sample into the beaker of boiling water the utility clamp was not working properly and forced my sample to touch the walls of the beaker. This could have effect my overall result.

Due to the relative size of mitochondria, the final pellet, Pellet 3, would most likely contain the most mitochondria. The second experiment used the competitive inhibitor, malonate, in different amounts, with the cell fraction created with pellet 3 from the previous experiment. The purpose was to see how malonate affected the absorbance at 600 nm. This inhibitor should slow down the conversion of succinate to fumarate. Due to the lower amount of conversion, there should be smaller change to the absorbance.

However, Tube E contained the same amount of glucose, but did not have the highest absorption value or quickest generation time. This is most likely due to the fact that Tube E did not contain phosphate buffer. Without the buffer, the acid produced by the bacteria’s fermentation may have lowered the pH of the solution

The Cycle of Cellular Respiration and Fermentation Introduction: In this Cellular Respiration and Fermentation lab, we discuss about the cellular respiration which is classified as an aerobic process and fermentation which is anaerobic process. An aerobic process is where it contains oxygen unlike the anaerobic process does not. In the aerobic process there are 4 steps that should be recognized: glycolysis, conversion, citric acid cycle, and the electron transport chain. This experiment contains or uses the yeast concentration to see how much CO2 developed over 20 minutes, counting per 2 minutes and measuring how much has progressed.

For the baseline experiment the hypothesis stated that there would be a positive exothermic reaction when the hydrogen peroxide was introduced to the yeast. There was a total increase in temperature for the baseline experiment was 1.9 degrees Celsius. The result shows that the hypothesis holds up and was indeed correct. This is because when the hydrogen peroxide was introduced to the hydrogen peroxidase (yeast) the enzyme bonded to the hydrogen peroxide and quickly decomposed the hydrogen peroxide.

Cellular Respiration is an aerobic process of molecules releasing stored energy in order to create ATP for oxygen. For a cell to complete this, oxidation-reduction reactions occur where it will break down an organic fuel. (Upadhyaya 39) In the absence of oxygen, fermentation occurs, consisting of two types: alcohol fermentation and lactic acid fermentation, in which both receive a hydrogen atom in order to form their necessary products. (Upadhyaya 40) In part one of this experiment, yeast was grown in either sucrose, glucose or a starch solution each at a temperature of either 37ºC, 25ºC or 4ºC. The hypothesis for this experiment was that the metabolic rate of yeast would be highest in glucose

There was not a control in this experiment. The independent variable is the type of carbohydrate and the dependent variable is the amount of CO2 produced or the rate of respiration. Some of the constants in this experiment is the amount of time the yeast and carbohydrate cured, the temperature of the water, and the amount of yeast and water solution mixed with the saccharide. If polysaccharides consist of more glucose, then more cellular respiration with occur with the yeast than the small saccharides. This was the hypothesis that a member of Mr.Woodruff’s sixth period class used in the

In this experiment, the subjects of study were fermentation, mitochondrial respiration, and redox reactions. In the first experiment, yeast was grown in various carbohydrate solutions at various temperatures. In the second experiment, succinate was added to various samples of a mitchondrial suspension, DPIP, and a buffer. Then after two blanks were used, the samples were placed into the spectrophotometer for transmittance testing.

In this lab, aerobic cellular respiration of germinating and dormant peas was measured using respirometers. Cellular respiration occur in the mitochondria, which the peas contain, through a series of reactions. Since all cells need energy, the primary source of cellular energy is carbohydrate, which are broken down into glucose to release energy, stored in adenosine triphosphate (ATP), from the bonds. Plants obtain glucose through photosynthesis while animals obtain it through consuming food. Cellular respiration can be summarized through the equation: C6H12O6 + 6 O2 --> 6 CO2 + 6 H2O + 36 ATP.

To understand this experiment, it is important to have a basic comprehension of what cellular respiration is, how it functions, and the different variables that can affect it. All organisms need some form of energy, taken from outside sources, to function and to facilitate growth and development. Mitochondria within cells are able to harvest the chemical energy stored in food molecules, such as glucose, amino acids, and fatty acids,  and break it down through chemical oxidation in cellular respiration. Cellular respiration is an ATP, adenosine triphosphate,  synthesizing process that also produces carbon dioxide and water as waste (Cellular, 2016). The reactions that take place in respiration are catabolic which means they break down complex molecules into simpler molecules while releasing energy. The energy released in this process is central to the production of ATP. ATP is a macromolecule consisting of a ribose, a nitrogenous base, and a chain of three phosphates. ATP is used to transport energy throughout the cell and releases the stored energy by breaking the bond on the outermost phosphate group (Bergman, 2015).

Cellular Respiration is a sequence of metabolic processes that occur in the microscopic cells of organisms to generate biochemical energy from nutrients into ATP and then discharge excess products. Cellular respiration can be simply understood as Organic Compounds + Oxygen → Carbon dioxide + Water + Energy. The reactions are C6H12O6 and 6 O2. The products are 6 CO2 + 6 H2O + Energy. The full equation for Cellular Respiration is C6H12O6 + Oxygen → 6 CO2 + 6 H2O + Energy (ATP + heat). If a substance loses electrons it is being oxidized. If electrons are being added to a substance it is being reduced. C6H12O6 and 6 CO2 becomes oxidized while 6 O2 and 6 H2O becomes reduced.

By changing the pH level of yeast, would it affect its cellular respiration? This experiment would prove that by looking into what conditions yeast best produces carbon dioxide (CO2). The solution of this experiment would be knowing what condition yeast grows best in, and then making it easier to produce more yeast around the world.