Black Worms Lab

An experiment was conducted to study and explore the circulatory system by exposing Lumbriculus variegatus, black worms, to household drugs. Lumbriculus variegatus was chosen as the experimental organism because of their transparent bodies and their simple physiology.

2. Pin the earthworm to the tray using one pin on either end of the worm.

Wet and Dry Environments To find whether Pill bugs gravitate toward a wet or a dry experiment, two coffee filters were cut to the size of the petri dish and placed inside, however one was dampened before being placed on the bottom. Dirt was then added on top to promote a more realistic environment and five Pill bugs were placed in each side before starting to prevent bias on an environment. A log was taken every thirty (30) seconds for ten (10) minutes on how many bugs were in each chamber and a graph was then made to show the changes of the bugs' positions over time. Again, if a Pill bug was in the transgression tunnel he was determined to be in a dish by the direction he was moving or the way it's head was facing.

One sowbug was then placed in the 3cm gap of the container and 30 seconds was started on the stopwatch. When time was completed we observed which side, grass or sand, the sowbug had preferred. Then, the sowbug was removed and a new one was placed in the container. The same steps were taken for each of the 20 bugs.

The experiment was conducted using six worms, where three were used for one treatment and the other three for a different treatment level. Initially, the worm was transferred to a parafilm slide containing some water and placed under a dissecting microscope. Application sticks were used to place the worm on the mark and an additional light was placed to observe. The focus was on the posterior end of the worm to obtain the basal pulsation rate. A timer was used to record the basal pulsation rate for 15 seconds, then the result was multiplied by 4 to get the number of beats per minute and this was repeated for two more trials. Same methods were used to measure the pulsation rate of two additional worms and the data were recorded.

As an experiment I would place multiple kinds of cereal in front of a single mealworm, and record their reactions to each certain type of

2. Place 10 randomly selected sowbugs in each of the 3 empty tin bowls for 3 minutes to allow them to acclimate to the environment.

For the first procedure the student put 5ml of distilled water in to a test tube then picked up one filter paper and placed it in a dish. The 5ml of distilled water was then dropped on to the filter paper. The student then grabbed alcohol for its habitat solution, and placed 5 ml of alcohol in to another test tube, and placed a new filter paper in to another clean dish. The 5ml of alcohol was then dropped on to the filter paper. Once both filter papers were submersed in the proper liquid then they were taken out of the dish and out in to individual chambers that had a connection. On the left hand side was the water filter paper and on the right hand side the alcohol filter paper. The second procedure was to grab ten pill bugs and gently place them in to the alcohol and water chamber.

This will help improve the understanding of pillbugs’ behavior as well as provide the students performing the experiment with more experience with lab work, and a better understanding of the scientific method.

We multiplied the 10 sec heart beat of the D. magna by 6 to get a total of one minute. After we finished with the water part (control group), we added alcohol to our slide and inspected the difference in the Daphnia magna’s heart beat through the microscope. We counted the new heart beat for 10 sec. We then turned the light off for 3 minutes to let the D. magna rest. We repeated this two more times (for a total of 3 times).

1.) Measure out 20ml out of the water and place it into a glass beaker

1 ml of water should be added to the first test tube and make a note. In the second test tube, 1 ml of methyl alcohol should be added. In the third test tube, 1 ml of hexane must be added. Lastly, the fourth test tube will be a control.

Ensure that all the flies are in the bottom area of the vial. 4. The flies will distribute themselves within the 3 compartments during a 20 second time period. Identify the number of flies in each compartment. 5.

Pour everything in the beaker into the evaporating dish, using a Popsicle stick to scrape the sand into the evaporating dish. 5. Put the wire gauze onto the ring stand and place the evaporating dish in the center of the wire gauze, then use a striker to light the Bunsen burner with a low flame and place the Bunsen burner on the base of the ring stand under the wire gauze, once the water boils, use beaker tongs to remove the evaporating dish from the ring stand and set it on the desk, then turn off the Bunsen burner. 6. Put the filter paper in the funnel and place the funnel over the 20 ml beaker and using the beaker tongs, carefully pour the salt and water from the evaporating dish into the funnel until there is mostly sand left in the dish and wait 3 minutes for all the salt and water to go through the funnel and into the 20 ml beaker, then take the filter out of the funnel and using the first Popsicle stick scrape off the melted stearic acid onto paper towel 3.

Nicotine, one of the most unusual psychoactive drugs known, and the primary pharmacological agent of addiction in cigarettes, triggers powerful physical and psychological reactions in species as diverse as cockroaches and humans.