FSQ 2204 - Experiment 1 parviz

PROCEDURES 1. Search at least five food products at home. 2. Determine the major fat component (Saturated or Unsaturated) RESULTS FOOD PRODUCTS French Fries fried using vegetable oil French Fries fried using Types of Fats Unsaturated Advantages/Disadvantages It has health benefits/It is costlier Saturated It is much cheaper/ Not healthy – fats will be deposited in veins derived from animal oil -- CONCLUSIONS

Susceptibility to spoilage is affected by water content of the food. Why? please explain very detail

Neonatal bilirubin samples are especially vulnerable to UV light degradation due to their small sample volume.   Question 9 options:   True   False

Name two more methods, besides the Bradford Assay, for determining protein concentration.

When patients are found to have increases or decreases in their total protein & albumin values, this test might be run next to narrow down the disease diagnosis:   Question 19 options:   serum protein electrophoresis   A/G ratio   glucose   cholesterol

Question: Why are we using this materials in the experiment is there any reason why we using it? Quantification of Total Protein in Serum Experiment; Materials: Fatty acid free beef albuminl solution: 100 g / L Reagent Ⅰ Sodium potassium tartrate 9g / LSodium hydroxide 0.2 mol / L         Potassium iodide 5 g / L Reagent Ⅱ Serum from blood   Copper sulphate solution: 150 g / L   Working solution: 5 ml of reagent copper sulphate solution is prepared by mixing into 1 L of reagent Ⅱ. This reagent is stable for 6 months at 2-8 ° C Procedure: *Three test tubes are taken. These are marked as blank, standard and sample tubes.*Only 5 ml of working solution is put into the blank tube.*0.1 ml reagent I and 5 ml working solution are put into the standard tube.*0.1 ml of serum and 5 ml of working solution are put into the sample tube.*After mixing each tube separately, it is waited for 30 minutes at room temperature and measured at 545 nm in the spectrophotometer.*Color permanence is…

✪ H₂N. ✪ H₂N H₂N H₂N ✪ H₂N ✪ H₂N NH₂ 0 H₂N 0 NH₂ ht Biglie O ✪ H₂N NH₂ NH₂ ·00 H₂N. H₂N NH₂ A OH CO₂H V8 Protease (cat) H,O, pH 7

The first step in the procedure asks you to prepare five dilutions of the protein standard (BSA) in the range of 0.2 to 1.0 mg/ml. For each concentration, a volume of 1.0 mL should be prepared. Describe how you will prepare these standards using a stock solution of BSA that is 2.0 mg/ml.

In replicate analyses, the carbohydrate content of a glycoprotein (a protein with sugars attached to it) is found to be 12.6, 11.9, 13.0, 12.7, and 12.5 g of carbohydrate per 100 g of protein. Find the 50% and 90% confidence intervals for the carbohydrate content.

describe the practical application of milk test

Which one of the following could be classified as a quantitative analysis?   Choices Isolation of omega-3 fatty acid from fish oil Determination of the omega-3 fatty acid content in olive oil Determination of fatty acid types in cooking oil Separation of fatty acid mixtures in guts of salmon

Antigen/Antibody (Ag/Ab) combination assays can detect HIV infection on average______ days earlier than third-generation antigen sandwich assays that only detect antibodies.     Question 7 options:   A)  five to seven   B)  fifteen to thirty   C)  one to two   D)  thirty to forty

Temperature 4 °C 21 °C 30 °C 37 °C 90 °C Absorbance at zero min 1.300 0.952 0.876 0.914 0.903 Absorbance after 10 min of incubation 1.160 0.743 0.042 0.016 0.758 Aomin – A10min 0.140 0.209 0.834 0.898 0.145 Enzyme activity in 10 min (umoles) Enzyme activity (umoles pyruvate/min) CALCULATIONS: show calculations Where extinction coefficient is 6.22 x 103

A1L TPN order is written for 400 mL 10% Dextrose, 300 mL 4.25% amino acids, and a total of 37 mL of additives. How much sterile water for injection should be added? a. 55 mL b. 137 mL c. 163 mL d. 263 mL

Complete the table:                                                                                     Test/ samples Glucose     Starch Molisch’s test   Purple ring     Fehling’s test Red precipitate Red precipitate No precipitate No precipitate Barfoed’s test   No precipitate No precipitate   Iodine test No colour   No colour Blue colour

Answered proteln structure 1 Which is an example of primary protein structure? Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer a. Multiple folded protein chains create an overall shape of a protein complex, b Amino acids form a particular sequence of residues A protein contains many alpha helices. A protein folds around itselrto exposehydrophilic groups on its surface

731375SSO Which of the following substances meets the definition of a pure "compound" according to the chemistry textbook? Place a check next to all choices that apply. O Distilled water: H20 O Pure copper metal: Cu O Dinitrogen Trioxide vapor: N203 OAmmonium Chloride: NH4CI O Himalayan Sea Salt: Mixture of NaCI, Fe203, and other trace minerals. O Compound W: (Read ingredients) Drug Facts Compound W FAST ACTING GEL PETt STAS

Give another suitable method that can analyze the protein content of a sample (egg yolk and egg white). Give reasons for your answer. Except Kjeldahl method

Give an example of product nutrition labels that disguise the label of "sugar" in the nutrition facts. Please state the term that been used. Give also the side effect of this hidder sugar label. Thank you!

Biochemistry: What does specificity constant mean?

Qualitative Test for Proteins

A phlebotomist gets a needlestick from a patient with known hepatitis B viral infection. The best immunoassay test to detect newly acquired disease in the phlebotomist is to measure the hepatitis B surface antigen within the first 3 months of the encounter.   Question 3 options:   True   False

A student is testing for the presence of protein, simple sugars, starch, and lipids using the tests summarized in the table below.  Molecule Test Positive Result Negative Result Protein Biuret reagent Pink or purple Blue Simple sugars Benedict's reagent Green, Orange or Red Blue Starch Iodine Orange, Blue or Purple Yellow Lipids Brown paper Oily Stain No stain   The student tests an unknown solution using each of the tests and observes that the solution turns blue-black when he tests for protein. Which of the following conclusions can the student make? A. The solution contains carbohydrates. B. The solution contains lipids. C. No conclusion can be drawn; the student made an error. D. The solution contains starch. E. The solution contains protein.

The image below is a positive ionization mode Electrospray ionization-mass spectrum (ESI-MS) spectrum for a peptide, obtained using high resolution mass spectrometry. If ionization occurs through de-protonation (assuming that the mass of a proton is 1.0 Da), what is the neutral mass of the peptide, in Da? 627.271 627.604 100 - 627.938 628.271 628.604 628.938 627.0 628.0 629.0 mass/charge (m/z) 628.271 626.271 1878.813 O 1884.813 % Abundance

You have learned quite a number of different physicochemical methods for fractionating the contents of cellular extracts and the proteins contained within them. Which technique would be the "best" to perform the following task. Removing K+ ions from a protein sample . Oion exchange salting out differential centrifugation O gel filtration O dialysis

How much protein do you need to add to the 800 µL buffer in the first microcentrifuge tube to make a 1:5 dilution? a) 500 µL b) 100 pl c) 100 mL d) 200 uL

Shown are the configuration of a gel electrophoresis experiment. Charged plates establish an electric field on a region of gel between the plates. A solution of DNA fragments, which acquire a negative charge in solution, is analyzed by using an electric field to drive the fragments through the gel. A drag force opposes the motion, causing the fragments to move at a constant speed. Larger fragments move more slowly, so the fragments separate by size to give a “fingerprint” of the sample. What is the energy transformation as the fragments move through the gel?A. Electric potential energy to kinetic energyB. Electric potential energy to thermal energyC. Electric potential energy to kinetic energy and thermal energyD. Kinetic energy to thermal energy

This pre-analytical variable can have an effect on chemistry tests which are read visually by spectrophotometry:   Question 8 options:   all answers are correct   icterus   lipemia   hemolysis

A solution contains alanine and serine. What are the possible dipeptide products that can form from this mixture? H H H H T HIL N- -O-H EN- H CH3 H CH₂OH serine (ser) alanine (ala) Enter each answer as two three-letter codes joined with a dash (e.g. ala-ala). Check Next (1 of 2) -O-H

Give the functions of the following ingredients, then name a branded/commercial skin or hair care product where the said material is used.  Tetrasodium EDTA  Cholesterol Silicone Oil 200/50 cst BETAINE Carbopol Ultrez D & C Red No. 17 ( CI No. 26100) Bisabolol (Alpha Bisabolol) Ceresin Wax Disodium Lauryl Sulfosuccinate Hydroxypropyl Methylcellulose

Now let us look at a real amino acid, alanine. Fill in the chart below for each ionizable group. You need only the relationship discussed in the pdf posted and the numbers posted below. The next 6 questions will ask for the numbers you calculated for each of the letters below. pH when Average charge of group Group Ka pKa [A]/[HA] = 1 when pH = pK alpha-COOH ~10-2 A C alpha-NH3* ~10-9 В

If Histidine, an amino acid with a basic "R", dissolves in H2O forming an aqueous (water) solution. One would expect that the aqueous solution would   a) change the pH toward 1  b) form covalent bonds  with the Glutamic acid, an  acidic amino acid c) form a crystal or solid d)  change the pH toward 14 e) become nonpolar

/evo/index.html?deploymentld=D55750828934189288909969212&elSBN=9781305657571&snapshotld=2199898&id%3D1.. O To * MINDTAP Q Search this ca Use the References to access important values if needed for this question. The amino acid glycine has the molecular formula C,H5NO2, and the amino acid isoleucine has the molecular formula CH13NO2. Determine how many moles of isoleucine contain the same number of atoms as 1.97 mol of glycine. moles • Submit Answer 5 question attempts remaining