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The City College of New York, CUNY *

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100

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Biology

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May 11, 2024

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pdf

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42

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Bio 229 Lab Final Exam 11 0 Name : Question 1 Answer ALL questions Score: 91/ 100 г # with * phosp hate backbon
e 2 2 2 V. IV . III . a . Define the following (5 x 2 Points = 10 Points) II . 1 . DNA Deoxyribonucleic
acid Double - stranded antiparallel sequence of nucleic acids that codes for RNA- > amino acids . Genetic code contained within all cells . Genomic DNA DNAD that codes for the entire genome. Present in all cells , but each cell type expresses a small portion of the gDNA different Single nucleotide polymorphism ( SNP) Point mutation in a single nucleotide .. common to $ 1 % of the general population Gel electrophoresis
In Protei n + biological mais molecules - RNA A method of separating DNA by weight DNA of Buffer it's electrophoresed from neg . to pos . charge and compared against a ladder to I see if the PCR contains a specific amplified in Sequence of amino acids bound by covalent peptide bonds . Forms Polypeptid e prod
uct of a protein . ~ 1 ~ of ↓ is preze nt . prim ary structure 7 b. List the necessary components (reagents) in a polymerase chain reaction (PCR ) . (4 Points) 1. Buffer ok 2. dNTPs not always . Template DNA 3. gDNA 4. Forward
primer 5. Reverse primer 6. Tag polymerase C. What are the main steps of a PCR ? ( Name each step) . You MUST include the temperature range in degrees Celsius (°C) for each step and explain why these temperatures are used. (4 Points) usually ~ 95° C 1. Denaturation ( '80 -100 ° C) 3 2 . -Unwind DNA -Hydrogen bonds between strands are broken at high temps
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