Exercise 23 Lab Report

ISOLATE #1 Results for "Isolate #1" ***Please fill out the table**** 1. Blood agar: 2. Nitrate Test: Test Results Interpretation Blood agar (type of hemolysis) Nitrate test 3. Gelatinase Activity: Gelatinase activity Resistant or Susceptible? Novobiocin Resistance Zone Diameter: 4. Novobiocin Resistance: 5. Coagulase Test: Coagulase (Sure-vue test) 1. Based on your results, what is the possible identification (Genus and species) of “Isolate #1"? I 9 10 11 12 3 14

ISOLATE #2 Results for "Isolate #2": ***Please fill out the table**** 1. Blood agar: 2. Nitrate Test: Test Results Interpretation Blood agar (type of hemolysis) Nitrate test 3. Gelatinase Activity: Gelatinase activity Resistant or Susceptible? Novobiocin Resistance Zone Diameter: 4. Novobiocin Resistance: 5. Coagulase Test: Coagulase (Sure-vue test) 2. Based on your results, what is the possible identification (Genus and species) of "Isolate #2"? nhes

General Instructions:Choose 1 bacteria/diseases and fill out the details. An example of an answered template is also provided for your reference.    Causative Agent and Disease Profile for S. aureus Template and Example   ITEM MSM PROFILE   MICROBIAL PROFILE   I MICROORGANISM/CAUSATIVE AGENT Staphylococcus aureus A GRAM REACTION (+) B OXYGEN REQUIREMENT Facultative Aerobes C SIZE 1.5 µm D SHAPE Cocci in clusters E HABITAT Normal flora of skin/anterior nares/pharynx F DISCOVERY   G MICROSCOPIC IMAGE   II DISEASE PROFILE Scalded skin syndrome A DISEASE/S                        Skin and Wound Infections       Scalded Skin Syndrome Toxic Shock Syndrome Food Poisoning Pneumonia B SYMPTOMS OF THE DISEASE A high fever · Nausea and vomiting · A rash on your palms and soles that resembles a sunburn C INCUBATION PERIOD 2 and 4 hours (range 30 minutes to 8 hours) D MODE OF…

BONUS (15 points) The fallowing series of dilution was prepared from a specimen to determine the number of bacteria. There were 62 colonies on the agar plate prepared by transferring 0.3 ml from the tube number 4. Calculate the dilution factors for each tube. What is the cell concentration in the original specimen? Calculate the total number of cells in tube number 2. étv A) F12 F9 F10 F7 % & %3D delete 5 { T Y J K

Case #2 Clinical History: This 29-year-old male's illness began 10 weeks prior to death, with an episode of "flu". Two weeks later his urine became "smoky". He was found to have hematuria, albuminuria and elevated BUN (180 mg/dl). He died from a pulmonary embolus. The throat culture obtained exhibited gram positive cocci in chains. It also showed beta- hemolysis on sheep blood agar (SBA) and was catalase negative. 1. What was the most likely organism? A. Streptococcus pyogenes B. Streptococcus viridans C. Staphylococcus aureus D. Streptococcus pneumonia 2. What is the BEST diagnosis at the time of death? 3. What are the other possible sequela of this infection?

LABORATORY REPORT: Aseptic Technique & Inoculation of Bacteria Guide Questions. 1. Why is direct flaming preferred when disinfecting loops and needles? 2. Why is it important to flame the entirety of the loop and not just the tip? What consequences can be seen when this process is not done correctly? 3. What is the difference between quadrant streak method A from method B? 4. Why do we use a pencil instead of a pen when labelling culture tubes or plates? Are there other alternatives in labelling? 5. Why is an inoculating needle used in Subculturing Techniques? Can a loop be used instead? NOTE: Please try to answer all of the question asked, i promised to give you a good ratings

Analysis of the pathological constituents GIVE THE FOLLOWING: - Name of Test/Test Reagent - Positive Result   1) Protein (Note: Presence of protein in urine is termed as proteinuria or albuminuria) A. Heat and Acetic acid test Fill a test about ¾ full of urine. Heat the upper portion gently to boiling for 1 -2 minutes being careful not to shake the tube. Rotate the tube to prevent overheating. A turbidity may be due to albumin, phosphates or carbonates. Add 3 drops of acetic acid drop by drop while boiling between each drop. If turbidity disappears, it is due to carbonates and phosphates. B. Heller’s nitric acid test Place 1 ml of urine sample in a test tube. Hold the test tube in an inclined position and carefully pour 1 drops of conc HNO3 down the side of the test tube. Note formation of white ring at the zone of contact of the two solutions which is an indication of the presence of protein.

Analysis of the pathological constituents GIVE THE FOLLOWING: a. Name of Test/Test Reagent b. Positive Result Blood (Note: Presence of blood in urine is termed as hemolobinuria) To 1 ml of freshly prepared Benzidine solution (sat’d sol’n of Benzidine in glacial Acetic acid) and 10 drops of urine sample. Add 5 drops of 3% H2O2. A positive result is indicated by a blue green color.

A 1.5-year-old child developed vomiting, diarrhea, and fever. Stool sample were inoculated into the Endo media. After 18 hours on the surface of the medium grew medium-sized, round, slightly convex red colonies with a metallic luster. The doctor suspected Escherichia coli. 1. Name the composition of the Endo agar media. 2. Describe the properties of bacterial colonies on the Endo media.  3. What purpose differential diagnostic Endo media used for?

amylasw test test for? medium+ - reagents   + Test/why?

Helping tags: Biology, bacterial count, dilution, serial dilution WILL UPVOTE, just pls help me answer the following questions and explain them clearly. Pls show complete solutions also for the computation part. Thank you. 1. A bacterial culture was grown for 9 hours. At 3-hour interval, the culture was sampled to determine the population of the culture, by transferring 25 ml of the suspension to 225 ml 0.85% NaCl. Three consecutive dilutions were further made by using 1 ml aliquot in 9 ml of 0.85% NaCI. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method. Sampling COUNTS 2nd dilution 3rd dilution 4th dilution 0,0 30: 35 250;245 1st dilution 1st 2nd 3rd 0; 0 240; 235 TNTC 55; 60 5; 6 TNTC TNCT TNTC TNTC a) Illustrate the dilution series used and label the final dilution of each dilution. b) Determine the bacterial count (CFU/ml) every 3 hours of incubation for 9 hours. Show all computations.

A. A Aa A 三,三,行,E AaBbCcDdE Aa AaBbCcDdF AaBbCcDdF AaBbCcDd A Normal No Spacing Heading 1 Heading 2 =y updates, fixes, and improvements, choose Check for Updates. 1. You have found a putative virus which is able to infect a bacterium causing increased mortality and resistance to all antibacterial agents. You have systematically purified the sample but have an unknown concentration of viruses. You perform the following serial dilutions. 0.5ml 1ml 5ml 0.1ml 2.5ml 1ml 15ml 1 3 6 7 Phage Buffer 100.5ml 9ml 14.5ml 99.9ml 25ml 5ml 985ml Tube Dilution in each Test Tube (Show Tube Dilution in each Test Tube (Show your your work) # # work) 4 1 6. O Focus glish (United States) W 30 DII DD 000 000 F4 80 F7 F8 F9 F10 F3 F5 F6 $4 & 4 5 6 8.

B. Microbial Processes Study the diagrams and answer the given questions. Pepartery For items no. 1 to 6, refer to the diagram at Glucose the left corner. 1. What process is shown in the diagram? 2. In what part of a bacterium does the process occur? 3. What are the raw materials of the process? Dnydryacetore phoghee (DHAP Enerr eenserving stage 2NAD NADH 4. What stage involves the consumption of two ATP molecules? 2000-0 synopen 5. What stage involves the production of four ATP molecules? Phoy PEP ZADP Pyuv ae

Matching Type Match the the condition/description to the correspondingtest/specimen to be conducted or evaluated. Choose the correct answer. Polymerase Chain Reaction Test Rapid Antibody Test Thyroxine Stimulating Hormone Blood typing Blood Chemistry Microscopic Stool Examination Complete Blood Count Physical Stool Examination Urinalysis Alanine Aminotransterase Level Susceptibility test Human Chorionic Gonadotropin Glycated Hemoglobin 1. Fasting Blood Sugar 2. Helminth Infestation 3. Presence of COVID-19 virus as antigen 4. Organ damage by Acetaminophen * 5. Antibiotic Resistance 6. Effectiveness of Metformin 7. Pregnancy 8. Ilicit drug use by bus drivers 9. Hypothyroidism 10.Identification of IgG and IgM against COVID-19 virus

Case Study: You are asked to inoculate lactose media (broth) with E. coli that was growing exponentially in glucose media (broth). You monitor the growth of these cells in the lactose broth using a viable plate count technique and plot the Growth Curve for E. coli growing c lactose. After a day of monitoring the cells, you note that the culture entered into lag phase growth initially and is now currently growing exponentially. In addition, you find that the cultures doubling time is 30 minutes. You know that the doubling time of E. coli in glucose media is 20 minutes. You did not change the oxygen content or temperature at Which you were growing the cells, E. coli is still aerobically respiring at 37°C. Question: If the lac operon is 'on' what does this imply about the cell? O The cell is dying and the lac operon is going to induce apoptosis. Apoptosis is truly cell death and the cells will enter into the death phase of the Growth Curve. O Lactose is being catabolized and glucose is no…

Please answer and explain TOPIC: STAINING FECAL SMEARS 1.) What are the advantages of staining fecal smears with permanent stains (namely: Iron hematoxylin stain, Wheatley's trichrome stain, and modified acid-fast stain)? Enumerate more than two and explain.

Case Study: You are asked to inoculate lactose media (broth) with E. coli that was growing exponentially in glucose media (broth). You monitor the growth of these cells in the lactose broth using a viable plate count technique and plot the Growth Curve for E. coli growing on lactose. After a day of monitoring the cells, you note that the culture entered into lag phase growth initially and is now currently growing exponentially. In addition, you find that the cultures doubling time is 30 minutes. You know that the doubling time of E. coli in glucose media is 20 minutes. You did not change the oxygen content or temperature at which you were growing the cells, E. coli is still aerobically respiring at 37°C. Question: If you want to grow E. coli as quick as possible would you grow it on lactose or glucose? O Mixing the two carbon sources would provide the most optimal growth conditions. O Lactose because of the generation time being faster. O Lactose and an anaerobic environment. O Glucose…

Case Study: You are asked to inoculate lactose media (broth) with E. coli that was growing exponentially in glucose media (broth). You monitor the growth of these cells in the lactose broth using a viable plate count technique and plot the Growth Curve for E. coli growing on lactose. After a day of monitoring the cells, you note that the culture entered into lag phase growth initially and is now currently growing exponentially. In addition, you find that the cultures doubling time is 30 minutes. You know that the doubling time of E. coli in glucose media is 20 minutes. You did not change the oxygen content or temperature at which you were growing the cells, E. coli is still aerobically respiring at 37°C. Question: In order to determine the doubling time of E. coli a viable plate countris performed. This means that you are truly counting O Dead cells only via a microscopic count O Live cells only via membrane filtration or serial dilution O All cells via membrane filtration or serial…

Identify whether the statements are TRUE or FALSE 1. You should use P-20 micropipettes to transfer volumes for spread plating. 2. You should use P-200 micropipettes to transfer volumes for pour plating.

Esc F1 F2 F3 F4 F5 F6 F7 F8 F9 F10 F11 40 %23 5. What is the physical appearance (solid, liquid, semi-solid) of the following media: broth tube, agar slant tube, agar deep tube, and agar plate. What are you testing when you introduce an inoculum with a needle on an agar deep tube? at

Procedure: 1. Prepare 5 test tubes. Place 1 ml of 1% starch and add 10 drops of saliva to each tube. Mix thoroughly. 2. Place the first tube in ice water, the 2nd tube leave at room temperature, the 3rd tube in 40°C , the 4th tube at 60°Cwater bath and the 5th tube boil for 2 minutes.. 3. Leave the 4 tubes in their respective temperatures for 30 minutes. The 4th tube allows to stand for 30 minutes after heating for 2 minutes. 4. Test the contents of each tube with iodine and benedict’s tests.

MATCHING TYPE. Match the phlebotomy materials found in Column A to the Collection Method found in Column B. Column A: Materials 1. Cotton 2. Plain glass tube3. Red top glass tube4. Gauge 23 syringe5. Two-way needle6. Adapter7. Lancet8. Lavender top plastic tube9. Tourniquet10. Micropore Column B: Collection MethodA. Syringe MethodB. Evacuated SystemC. Winged CollectionD. Capillary CollectionE. All of the aboveF. Syringe and Evacuated onlyG. Evacuated and Winged

No SIM ? 10:33 PM 60% السؤال Staphalococcus is differed from Stretpococcus by: Catalse test O Coagulase test O Gram negative O Phosphatease حفظ الإجابة

Wil completed a full plate titer assay and there were 25 plaques on his 10^-7 plate. What is the titer of his high titer lysate? 10uL of each dilution were used for the plaque assays. O 2.5X10^11 pfu/mL O 2.5X10^7pfu/mL O 2.5X10^9 pfu/mL O 2.5X10^8 pfu/mL O 2.5X10^10 pfu/mL

Area of no growth around an antibiotic disk indicates_________________.

Plasma and clot e) Serum and clot 14. If small clots are noted when making a blood smear, one should: a) Make the smear and note on the slide that clots are present Mix the blood specimen for one hour then make the smear Do nothing; small clots will not affect a blood smear Request a new specimen before doing the blood smear Add more anticoagulant to the blood tube, mix, then make the blood smear 15. What is the purpose of doing a differential? a) b) To determine the proportion of RBCS in the whole blood To count the number of WBC's in whole blood To determine the proportion of WBC's in whole blood To microscopically examine RBCS and platelets To diagnose anaemia