Bio 131 Wk 10_REC_ Translation_Activity_STUDENT_Fa2023

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Drexel University *

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Biology

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May 8, 2024

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Bio 131 Week 9 (10) Translation – Ch 14 Gene Expression Recitation Active Learning Activity 1.) Label the following diagram 1.) DNA 2.) Nuclear Membrane 3.) Transcription 4.) mRNA 5.) Nuclear pore 6.) RNA modification 7.) Polypeptide chain 8.) Ribosome 9.) Codon 10.)Anticodon 11.)tRNA 12.)Amino-Acid 13.)Modified mRNA #13 #12 #11 #9 #10 #8 #7 #6 #5 #4 #3 #2 #1
For Component #8, what three sites would be found in this structure? o What site would the 1 st tRNA enter? P site o What would be the codon at this site? AUG o What would be the amino acid coded? Methionine o What would the anti-codon be? Mark its polarity! UTC o What site would all other tRNAs enter? A site o For the growing polypeptide chain from what site to what site does it transfer? From A site to P site For Component #8 in what direction does this structure move? 5’ to 3’ Fill-in the blanks for the step-wise process of creating Component #8: o Small Ribosomal subunit binds mRNA. o An initiator tRNA with the anticodon UAC base pairs with the start codon AUG . o The Large ribosomal subunit joins the initiation complex with tRNA at the P site. o Hydrolysis of GTP provides the energy for assembly. Why do antibiotics like Erythromycin, Chloramphenicol and Aminoglycosides that inhibit protein synthesis effect bacteria but don’t harm the host cell? Answer: They likely affect the ribosomes present in bacterial cells. Since eukaryotic ribosomes are 80s while prokaryotic ribosomes are 70s, they are structurally different. The antibiotics are likely targeted towards 70s ribosomes and hence do not affect protein synthesis in eukaryotes. Using the codon chart, complete the following: o Template DNA: 3’ TAC CTT CGG 5’ o Non-template DNA: 5’ ATG GAA GCC 3’ o mRNA: 5’ AUG GAA GCC 3’ o Polypeptide: Met Glu Ala Use the polypeptide three letter designation as your password for today’s Rec session. Please write the password in the following format: Xxx/Xxx/Xxx If the 3’ CG G 5’ in the template DNA strand above was mutated to 3’ CG C 5’, what would the sequence of the non-template DNA, mRNA and amino acid sequence be?
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